EC Number   |
|---|
   2.7.7.87 | a polycistronic sequence containing the genes encoding the PaKEOPS protein subunits Kae, Bud32, Cgi121 and Pcc1 synthesized and cloned into pET28(a) plasmid and transformed in Escherichia coli, Pcc1 fused to a hexa-histidine tag, PaSua5 cloned into pET26b |
| 2.7.7.87 | Escherichia coli BL21(DE3) cells transformed with a pET21 derivative (Novagen) vector containing the Escherichia coli yrdC gene |
| 2.7.7.87 | full length Sua5 expressed in Escherichia coli BL21 (DE3) CodonPlus cells harboring an N-terminal hexa-histidine tag |
| 2.7.7.87 | His6-tagged YrdC protein overexpressed in Escherichia coli |
| 2.7.7.87 | ScSua5 gene amplified by PCR using genomic DNA from Saccharomyces cerevisiae, fused to a hexa-histidine tag at its 3'-end and cloned into pET21d vector and transformed in Escherichia coli |
| 2.7.7.87 | SDS-PAGE, cloning of ywlC and ydiB as His6 N-fusion proteins and highly expression in Escherichia coli BL21(DE3), because of inclusion bodies construction of N-terminal thioredoxin (trxA) fusion proteins of ydiC and ydiE, which allow good expression and sufficient solubility for in vitro studies |
| 2.7.7.87 | Sulfolobus tokodaii Sua5 protein overexpressed in Escherichia coli |
| 2.7.7.87 | yrdC, yjeE and yeaZ genes from Escherichia coli expressed as N-terminal His6-fusion proteins, YgjD N-terminal as His6-SUMO fusion protein in Escherichia coli |
