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EC Number
cloned into pMAL-c4X vector and expressed in Escherichia coli Origami B (DE3) cells as an N-maltose binding protein (MBP)-tagged and C-His6-tagged fusion protein
cloning and sequencing of cDNA
cloning and sequencing of the full-length cDNA
comparison of sequences of enzyme from placenta and HeLa cells
DNA and amino acid sequence determination and analysis, promoter activity analysis
DNA fragments coding the catalytic domain (Cd7) sequence (residues 71-322) and the C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) are cloned into a pET23a vector and expressed in Escherichia coli
enzymatically active soluble N-deglycosylated enzyme form
enzyme fused to protein A is expressed as a soluble form in COS-7 cells
expression as GFP-tagged enzyme in HeLa cells
expression in Cos-1 cells
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