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EC Number
Commentary
Reference
2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli; coexpression of genes D1 and D12 from 1 plasmid; vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21
2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli; vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21
as a His6-Smt3 fusion protein in Escherichia coli BL21(DE3) CodonPlus RIL using a pET-based pSmt3-TOPO vector
complementation of knock-down HeLa S3 cells with enzymatically active N-truncated enzyme comprising residues 120-476 and being located at the nucleus, no complementation of knock-down HeLa S3 cells with enzymatically active N-truncated enzyme comprising residues 144-476 and being loacetd mainly in the cytoplasm, truncated enzymes are expresss in fusion with GFP
D1 fragment, amino acids 498 to 844; expression of D1 fragment mutants in Escherichia coli; individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli
D1 fragment, amino acids 498 to 844; individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli
D1 fragment, amino acids 520-844; individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli
expressed in Saccharomyces cerevisiae strains YBS40, YBS2, and YBS20
expression in Escherichia coli, His-tagged; expression in Escherichia coli, His-tagged, amino acid sequence analysis; expression in Escherichia coli, His-tagged, amino acid sequence analysis
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