EC Number   |
|---|
   2.1.1.189 | expression in Escherichia coli |
| 2.1.1.189 | expression of a intein-fusion protein with an N-terminal His-tag |
| 2.1.1.189 | gene PH1259, cloning, and DNA and amino acid sequence determination, phylogenetic analysis of PH1259 and its homologues, the gene PH1259 likely originates from a single horizontal gene transfer (HGT) event from Gram positive bacteria (RlmCD) rather than Gram-negative bacteria (RlmD or RumA), thus, it is referred to as PhRlmCD. Recombinant expression of His-tagged enzyme in Escherichia coli |
| 2.1.1.189 | gene yefA, phylogenetic analysis, functional complementation of Bacillus subtilis knockout strain BG12826 yefA::pMutin2 and DELTAyefA::Cm deletion mutant, and of Escherichia coli enzyme-deficient BW 25113 DELTAtrmA/DELTArlmC/DELTArlmD strain |
