EC Number   |
Reference  |
|---|
    1.8.4.9 | - |
288640, 288641, 288642, 288643, 288644, 668650 |
| 1.8.4.9 | a heterologous system is constructed in which the C domain of EiAPR was fused to the carboxyl terminus of the APS reductase from Pseudomonas aeruginosa (PaAPR), an enzyme that normally uses thioredoxin as an electron donor and is incapable of using glutathione for this function. The hybrid enzyme, which retains the [4Fe-4S] cluster from PaAPR can use both thioredoxin and glutathione as an electron donor for 5'-adenylylsulfate reduction. Expression in Escherichia coli |
667746 |
| 1.8.4.9 | APR, DNA and amino acid sequence determination and analysis, semiquantitative expression analysis, overexpression of His-tagged APR isozyme in Escherichia coli |
694695 |
| 1.8.4.9 | expressed in Escherichia coli strain BL21(DE3)-pLysS |
667703 |
| 1.8.4.9 | expression in Escherichia coli |
689431 |
| 1.8.4.9 | expression of N-terminally His-tagged PpAPR in Escherichia coli, expression of GFP-tagged APR in Physcomitrella patens plants using the mAV4 vector containing the chloroplast transit peptide from Physcomitrella patens FtsZ2-1 and transient transfection of protoplasts |
687585 |
| 1.8.4.9 | gene APR1, expression of the holoenzyme APR1p in Escherichia coli strain BL21(DE3), separate expression in Escherichia coli strain BL21(DE3) of amino acid residues 73-327, forming the R-domain, and of residues 328-465, forming the C-domain, the domains alone are inactive, but mixing of both can partially restore activity |
660399 |
| 1.8.4.9 | gene APR2, quantitative real-time PCR isozyme expression analysis, analysis of transcript profiles of APR genes in Arabidopsis plants under Cd stress, changes in transcript levels of APR1, APR2, and APR3 at various time points in response to Cd exposure in Arabidopsis thaliana plants, overview. Transgenic overexpression of APR2 in Arabidopsis thaliana plants by Agrobacterium tumefaciens strain GV3101 transfection method |
764564 |
| 1.8.4.9 | overexpression in Escherichia coli |
687585 |
| 1.8.4.9 | recombinant expression of N-terminally GST-tagged C-terminal redox domain (amino acids 353-461) of AtAPR1 in Escherichia coli strain BL21(DE3) |
763921 |
