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EC Number
Commentary
Reference
amplification of the structural torA gene encoding the enzyme and iits flanking regions using PCR techniques, the torA gene is the third gene of a TMAO-inducible operon, torECAD, encoding the TMAO respiratory components, the torC gene encodes a pentahemic c-type cytochrome, likely to be involved in electron transfer to the TorA terminal reductase, the role of the torE gene is unknown, torD gene encodes the TorD protein, a cytoplasmic protein involved in the folding process of the TorA precursor protein
expressed in Escherichia coli
expressed in Escherichia coli; overexpression as His-tagged protein
expression in strain BL21(DE3) as His-tagged protein
fusion protein with beta-galactosidase
gene torA, the torCAD operon encodes the Tor system induced in the presence of trimethylamine oxide mainly during exponential phase
gene torD, DNA sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) as His-tagged protein
GFP-tagged enzyme expression, the genome sequence of Vibrio fischeri reveals three putative TMAO reductase operons, transcriptional regulation, overview
overexpression
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