cloned into plasmid containing the sequence coding for HO1 protein
coexpression in Escherichia coli BL21(DE3) with cpcB(C155I) or pecB(C155I) and with cpeS, ho1 and pcyA, resulting in biosynthesis of the respective beta-subunits singly chromophorylated at cysteine-beta84
expression in Arabidopsis thaliana
expression in Escherichia coli
expression of mutant enzymes in Escherichia coli strain BL21(DE3)
gene pycA, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
into vector pET-21a and expressed in Escherichia coli C41(DE3)
PcyA expression in Escherichia coli strain BL21(DE3)
recombinant expression of GST-tagged PcyA in Escherichia coli