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Results 1 - 10 of 11 > >>
EC Number
Commentary
Reference
DNA and amin acid sequence determination and analysis, sequence comparisons
gene CHL27, encoding the CHLH subunit of enzyme Mg protoporphyrin monomethylester cyclase
gene OsCRD1, encoding a putative subunit of enzyme MPEC, is a single-copy gene in rice, sequence comparisons, quantitative real-time RT-PCR enzyme expression analysis
gene PeMPEC, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant overexpression in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV310 transformation method. Gene PeMPEC driven by the CAMV 35S promoter is transferred into the Crd1 mutant leading to functional complementation of the mutant, the chlorophyll concentration of sense plants is 22-50% higher than that of the Crd1 mutant, and 7-20% higher than that of wild-type Col-0
gene xantha-1, DNA and amino acid sequence determination and analysis
gene xantha-1, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in transgenic barley plants
gene ygl8 encodes a catalytic subunit of MgPME cyclase, DNA and amino acid sequence determination and analysis, map-based cloning, sequence comparisons and phylogenetic analysis. Transient expression in Nicotiana benthamiana. The recombinant construct pC1305-YGL8 is introduced into the ygl8 rice mutant by Agrobacterium tumefaciens EHA105-mediated transformation. Quantitative real-time PCR expression analysis
genes chlAI, chlAII, slr0905, sll1242, or slr0309, expression of wild-type and mutants enzymes, expression analysis, overview
PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method
RNA is isolated to synthesize cDNA and quantitative real-time PCR is carried out for anaerobic and semiaerobic growth conditions
Results 1 - 10 of 11 > >>