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Results 1 - 6 of 6
EC Number
Commentary
Reference
DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21(DE3)Gold
expression in Escherichia coli, fusion protein with green fluorescent protein and with His-tag
expression of either the wild type JBP2 or the mutant JBP2 proteins in insect stage trypanosomes, which do not contain the modified base J and determined the base J content of each cell line, mutations made within the key catalytic domain ablates the ability of JBP2 to initiate de novo base J synthesis
JBP2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes, the latter contain 4 exchanges of conserved residues
recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Codon Plus
Results 1 - 6 of 6