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EC Number
expressed in Escherichia coli strain BL21(DE3)pLysS
expression in Escherichia coli
transient expression of GFP-acireductone dioxygenase 1 in Arabidopsis thaliana protoplasts. The coding regions of ARD1–4 are cloned into pDEST17TM and pDEST15TM containing an N-terminal poly-His tag and an N-terminal GST tag, respectively, and are expressed in Escherichia coli strain Rosetta BL21
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