EC Number   |
Reference |
|---|
   2.4.1.90 | histidine-tagged 46000 Da protein produced in Escherichia coli |
489542 |
| 2.4.1.90 | isolation and characterization of the genomic locus |
489506 |
| 2.4.1.90 | isolation of a cDNA clone that encodes a major portion of galactosyltransferase |
489499 |
| 2.4.1.90 | molecular cloning and nucleotide sequencing |
489507 |
| 2.4.1.90 | NmLgtB is cloned into pET15b vector and expressed in Escherichia coli BL21 (DE3) cells as N-terminal His6-tagged fusion protein. Under common expression conditions only a low amount of soluble active enzyme is obtained. The expression level is improved 15fold to 12.5 mg/L culture under osmotic stress condition using LB medium supplemented with 0.2% glucose and a higher concentration of NaCl |
719274 |
| 2.4.1.90 | quantitative enzyme expression analysis in strains UR6 and AG83, overview |
686983 |
| 2.4.1.90 | recombinant enzyme is N-glycosylated |
489508 |
| 2.4.1.90 | transient functional overexpression in 7721 hepatocarcinoma cells |
659925 |
