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EC Number	Cloned (Commentary)	Reference
2.1.1.179	phylogenetic analysis, sequence comparisons	719353
2.1.1.179	sgm is cloned into a yeast expression vector to test whether the prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to the aminoglycoside antibiotic G-418. Sgm does not provide resistant phenotypes to yeast cells. Despite all similarities in the antibiotic binding site, methylation by Sgm does not occur in yeast, suggesting that the recognition site for these methylases could be different in 30S and 40S subunits	706701

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Release 2023.1 (January 2023)