EC Number   |
Reference |
|---|
    1.3.98.1 | genes subcloned int the EcoRI site of pUC18, PCR products cloned in vector pET100/D-TOPO, recombinant DHOD1 and DHOD2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RP |
676289 |
| 1.3.98.1 | into vector pET-28a and overexpressed in Escherichia coli strain BL21(DE3) as a histidine-tag fusion protein |
676964 |
| 1.3.98.1 | into vector pRSET and expressed in either freshly transformed or glycerol stocked Escherichia coli BL21-CodonPlus (DE3)-RIL |
671050 |
| 1.3.98.1 | ligated into the pZErO-2 vector and transformation of the Escherichia coli strain TOP 10, inserted into the pET3a expression vector and transformation of Escherichia coli BL21 |
671086 |
| 1.3.98.1 | transformation in Escherichia coli for detection of pyrimidine prototrophic colonies with a resistance of ampicillin |
390916 |
