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expression under the control of a rhamnose-inducible promoter in Escherichia coli strain JM109
recombinant functional expression of the enzyme in the cell envelope of Escherichia coli, where it is accessible for externally added protease
The nitrilase gene from Pseudomonas putida is cloned in plasmid pET 21b(+) and over-expressed as histidine-tagged protein in Escherichia coli strain BL21 (DE3)
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