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EC Number
Commentary
Reference
expression of the enzyme in Trp-auxotrophic Escherichia coli, resulting in site-specifically replacement of Trp residues with (2,7-aza)Trp
for expression in Escherichia coli BL21DE3-Gold cells
fusion of the antiferritin antibody VL domain to barnase and expression in Escherichia coli
gene rng, overexpression of wild-type rng and of mutant rngs in rne-1 or rneD1018 alleles mutant strains
genetic organization, low level expression of barnase by recombinant Bacillus subtilis strains bearing phoPR-null mutations
genetic organization, production of binase by recombinant Bacillus subtilis strains bearing phoPR-null mutations is impossible
into the pGEM-T vector for transformation of Escherichia coli JM109 cells, into pPIC9K for expression in Pichia pastoris
into the pQE30 vector for expression in Escherichia coli M15 cells
overexpression in Neurospora crassa, different promoters are tested, the most promising promoter for recombinant expression is the cfp promoter
overexpression in Saccharomyces cerevisiae. Characterization of an rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae. The rns4 mutant is sensitive to both RNase T1 and ambient stress. In contrast to the wild-type strain in which the inactivated RNase T1-GFP fusion protein is localized at the vacuole only under cold stress or nitrogen starvation, the inactivated RNase T1-GFP fusion protein expressed in the rns4 mutant is localized at the ER and vacuole, both under normal growth conditions and upon ambient stress conditions
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