EC Number |
Subunits |
Reference |
---|
4.2.3.4 | ? |
x * 37397, electrospray ionisation mass spectrometry |
716413 |
4.2.3.4 | ? |
x * 38888, electrospray ionisation mass spectrometry |
716413 |
4.2.3.4 | ? |
x * 39000, SDS-PAGE |
763080 |
4.2.3.4 | ? |
x * 45189, electrospray ionisation mass spectrometry, His-tagged protein |
716413 |
4.2.3.4 | dimer |
2 * 165000, SDS-electrophoresis |
137638 |
4.2.3.4 | dimer |
2 * 41000, SDS-PAGE of recombinant His-tagged enzyme |
-, 763209 |
4.2.3.4 | dimer |
2 * 41097, mass spectrometry, 2 * 41098, calculated |
729105 |
4.2.3.4 | dimer |
electron density map reveals 2 molecules (AB) per asymmetric unit. Disordered regions include the N-terminal 1-3 segment and 2 loop regions. The electron density map shows the presence of a piece of density in each subunit, which could be modeled as a zinc ion in each subunit. Subunits A and B associate into an AB dimer related by a non-crystallographic two-fold axis, and three AB dimers assemble into a hexamer along the 3-fold axis |
690739 |
4.2.3.4 | homodimer |
2 * 37397, electrospray ionisation mass spectrometry |
722147 |
4.2.3.4 | homodimer |
dynamic light scattering with 1 mg/ml protein at pH 8.0, 18°C in 20 mM Tris-HCl buffer |
-, 666871 |