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Results 1 - 6 of 6

EC Number	Substrates	Commentary Substrates	Organism	Products	Commentary (Products)	Reversibility
5.4.99.33	(3S)-2,3-epoxy-2,3-dihydrosqualene	-	Cucurbita pepo	cucurbitadienol	-	?
5.4.99.33	(3S)-2,3-epoxy-2,3-dihydrosqualene	-	Siraitia grosvenorii	cucurbitadienol	-	?
5.4.99.33	(3S)-2,3-epoxy-2,3-dihydrosqualene	-	Citrullus colocynthis	cucurbitadienol	-	?
5.4.99.33	(S)-squalene-2,3-epoxide	-	Cucurbita pepo	cucurbitadienol	-	?
5.4.99.33	more	GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol	Siraitia grosvenorii	?	-	?
5.4.99.33	more	11-oxo-24,25-epoxy cucurbitadienol might be a derivative of 24,25-epoxy cucurbitadienol, which is assumed to be formed through cyclization of 2,3-(S)-22,23-(S)-dioxidosqualene in yeast cells by recombinant SgCbQ, and then sequentially oxidized by recombinant CYP87D18 (EC 1.14.14.71) at the C-11 position in BY4741-Z5 as the oxidation process of cucurbitadienol. Metabolite identification by TLC, metabolite structures are confirmed by liquid chromatography-solid-phase extraction-nuclear magnetic resonance-mass spectrometry coupling (LC-SPE-NMR-MS)	Siraitia grosvenorii	?	-	?

Results 1 - 6 of 6

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Release 2023.1 (January 2023)