EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  4.2.3.127 | (2E,6E)-farnesyl diphosphate |
- |
Coprinopsis cinerea |
beta-copaene + diphosphate |
- |
? |
| 4.2.3.127 | (2E,6E)-farnesyl diphosphate |
- |
Cananga odorata var. fruticosa |
beta-copaene + diphosphate |
- |
? |
| 4.2.3.127 | (2E,6E)-farnesyl diphosphate |
Cop4 produces 10 different sesquiterpene products with delta-cadinene and beta-copaene as the major products in vivo |
Coprinopsis cinerea |
beta-copaene + diphosphate |
- |
? |
| 4.2.3.127 | (2E,6E)-farnesyl diphosphate |
conversion of (E,E)-farnesyl diphosphate proceeds via an (E,E)-germacradienyl carbocation in the case of Cop4. Cyclization of all-trans-farnesyl diphosphate compared to the cyclization of the cis-trans isomer of farnesyl diphosphate serving as a surrogate for the secondary cisoid neryl cation intermediate generated by sesquiterpene synthases capable of isomerizing the C2-C3 bond of all-trans-farnesyl diphosphate. (Z,E)-FPP is cyclized via a (6S)-beta-bisabolene carbocation by Cop4 |
Coprinopsis cinerea |
beta-copaene + diphosphate |
- |
? |
| 4.2.3.127 | (E)-geranyl diphosphate |
the enzyme accepts (E)-geranyl diphosphate as a substrate, but the catalytic efficiency with the shorter prenyl-diphosphate substrate is lower compared to their longer farnesyl diphosphate substrate |
Coprinopsis cinerea |
? |
- |
? |
| 4.2.3.127 | more |
Cop4 synthesizes delta-cadinene as its major product, cf. EC 4.2.3.13. Cop4 cultures produce several sesquiterpene compounds betacubebene, sativene, beta-copaene, and cubebol |
Coprinopsis cinerea |
? |
- |
? |
| 4.2.3.127 | more |
Cop4 is a catalytically promiscuous enzyme that cyclizes (E,E)-farnesyl diphosphate into multiple products, including (-)-germacrene D and cubebol. But changing the pH of the reaction drastically alters the fidelity of Cop4 and makes it a highly selective enzyme |
Coprinopsis cinerea |
? |
- |
? |
| 4.2.3.127 | more |
Escherichia coli strains expressing Cop4 accumulate delta-cadinene as the major terpenoid, accounting for about 40% of the total sesquiterpenes detected |
Coprinopsis cinerea |
? |
- |
? |
| 4.2.3.127 | more |
enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene |
Cananga odorata var. fruticosa |
? |
- |
? |
| 4.2.3.127 | more |
Cop4 synthesizes delta-cadinene as its major product, cf. EC 4.2.3.13. Cop4 cultures produce several sesquiterpene compounds betacubebene, sativene, beta-copaene, and cubebol |
Coprinopsis cinerea ATCC MYA-4618 |
? |
- |
? |
