EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
    4.2.3.103 | ent-copalyl diphosphate |
- |
Oryza rufipogon |
ent-isokaurene + diphosphate |
- |
? |
| 4.2.3.103 | ent-copalyl diphosphate |
- |
Oryza sativa Japonica Group |
ent-isokaurene + diphosphate |
- |
? |
| 4.2.3.103 | ent-copalyl diphosphate |
- |
Oryza sativa Indica Group |
ent-isokaurene + diphosphate |
- |
? |
| 4.2.3.103 | ent-copalyl diphosphate |
more than 90% yield |
Oryza sativa Indica Group |
ent-isokaurene + diphosphate |
- |
? |
| 4.2.3.103 | ent-copalyl diphosphate |
- |
Oryza sativa Indica Group |
ent-isokaur-15-ene + diphosphate |
- |
? |
| 4.2.3.103 | more |
GC-MS reaction product analysis. The distinct function of two alleles of a KSL from rice, OsKSL5i and OsKSLj, identifies a single residue switch with a profound effect on not only OsKSL5 product outcome but also that of land plant KSs more broadly, specifically, replacement of a key isoleucine with threonine, which interrupts formation of the tetracyclic ent-isokaurene at the tricyclic stage, leading to production of ent-pimaradiene instead |
Oryza sativa Indica Group |
? |
- |
? |
| 4.2.3.103 | more |
recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene |
Oryza rufipogon |
? |
- |
? |
