EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
4.2.2.24 | alpha-L-Rha-(1,4)-beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA |
- |
Penicillium chrysogenum |
? |
- |
? |
4.2.2.24 | beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA |
- |
Penicillium chrysogenum |
? |
- |
? |
4.2.2.24 | beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA-(1,2)-alpha-L-Rha |
- |
Penicillium chrysogenum |
? |
- |
? |
4.2.2.24 | beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA-(1,2)-alpha-L-Rha-(1,4)-beta-D-GalA |
- |
Penicillium chrysogenum |
? |
- |
? |
4.2.2.24 | more |
enzyme is an exo-type polysaccharide lyase that cleaves rhamnogalacturonan from the reducing end. The active site possesses an L-shaped cleft that can accommodate galactosyl side chains |
Penicillium chrysogenum |
? |
- |
? |
4.2.2.24 | rhamnogalacturonan I |
responsible for an initial cleavage of the rhamnogalacturonan I region of plant cell wall pectin. Bacillus subtilis strain 168 secretes two rhamnogalacturonan lyases, YesW and YesX, extracellularly. YesW cleaves the glycoside bond of the rhamnogalacturonan chain endolytically, and the resultant oligosaccharides are subsequently converted to disaccharides, unsaturated galacturonyl rhamnose, through the exotype reaction of YesX |
Bacillus subtilis |
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end |
- |
? |
4.2.2.24 | rhamnogalacturonan I oligosaccharide |
the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR |
Bacillus subtilis |
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end |
- |
? |
4.2.2.24 | rhamnogalacturonan I oligosaccharide |
rhamnogalacturonan I from potatoes. YesX acts on the substrate exolytically and releases disaccharides as major product. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan |
Bacillus subtilis |
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end |
- |
? |
4.2.2.24 | rhamnogalacturonan I oligosaccharide |
the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR |
Bacillus subtilis 168 |
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end |
- |
? |
4.2.2.24 | rhamnogalacturonan I oligosaccharide |
rhamnogalacturonan I from potatoes. YesX acts on the substrate exolytically and releases disaccharides as major product. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan |
Bacillus subtilis 168 |
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end |
- |
? |