EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  4.2.1.168 | GDP-4-dehydro-alpha-D-rhamnose + L-glutamate |
- |
Escherichia coli |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + 2-oxoglutarate + ammonia |
- |
? |
| 4.2.1.168 | GDP-4-dehydro-alpha-D-rhamnose + L-glutamate |
- |
Yersinia pseudotuberculosis |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + 2-oxoglutarate + ammonia |
- |
? |
| 4.2.1.168 | GDP-4-dehydro-alpha-D-rhamnose + L-glutamate |
- |
Escherichia coli |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + 2-oxoglutarate + ammonia |
overall reaction |
? |
| 4.2.1.168 | GDP-D-perosamine + L-glutamate |
- |
Escherichia coli |
GDP-4-dehydro-3,6-dideoxymannose + 2-oxoglutarate + ammonia |
wild-type ColD is able to catalyze the production of GDP-4-keto-3,6-dideoxymannose using GDP-perosamine instead of GDP-4-keto-6-deoxymannose as a substrate |
? |
| 4.2.1.168 | more |
enzyme functions as GDP-4-keto-6-deoxy-D-mannose-3-dehydrase responsible for C-3 deoxygenation of GDP-4-keto-6-deoxy-D-mannose. The enzyme is coenzyme B6-dependent and catalysis is initiated by a transamination step in which pyridoxal 5'-phosphate is converted to pyridoxamine 5'-phosphate in the presene of L-glutamate. This coenzyme forms a Schiff base with the keto sugar substrate and the resulting adduct undergoes a pyridoxamine 5'-phosphate-mediated beta-dehydration reaction to give a sugar enamine intermediate, which after tautomerization and hydrolysis to release ammonia yields GDP-4-keto-3,6-dideoxy-D-mannose as the product |
Yersinia pseudotuberculosis |
? |
- |
? |
