EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O |
- |
Streptomyces coelicolor |
(-)-geosmin + acetone |
- |
? |
| 4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O |
- |
Sorangium cellulosum |
(-)-geosmin + acetone |
- |
? |
| 4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O |
- |
Aphanizomenon gracile |
(-)-geosmin + acetone |
- |
? |
| 4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O |
catalysed by the C-terminal domain of the bifunctional enzyme |
Streptomyces coelicolor |
(-)-geosmin + acetone |
- |
? |
| 4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O |
- |
Sorangium cellulosum Soce56 |
(-)-geosmin + acetone |
- |
? |
| 4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O |
- |
Streptomyces coelicolor ATCC BAA-471 / A3(2) / M145 |
(-)-geosmin + acetone |
- |
? |
| 4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O |
- |
Aphanizomenon gracile WH-1 |
(-)-geosmin + acetone |
- |
? |
| 4.1.99.16 | (2E,6E)-farnesyl diphosphate + H2O |
overall reaction of germacradienol/geosmin synthase |
Streptomyces avermitilis |
(4S,7R)-germacra-1(10)E,5E-diene-11-ol + (7S)-germacrene D + geosmin + ? |
presence of Mg2+, synthesis of 66% (4S,7R)-germacra-1(10)E,5E-diene-11-ol, 24% (7S)-germacrene D, 8% geosmin, and 2% of a hydrocarbon, tentatively assigned the structure of octalin |
? |
| 4.1.99.16 | more |
conversion of germacradienol to geosmin results in the release of the three-carbon side chain as acetone and involves a 1,2-hydride shift of the bridgehead hydrogen exclusively into ring B of geosmin |
Streptomyces coelicolor |
? |
- |
? |
| 4.1.99.16 | more |
nerolidyl diphosphate is excluded as an intermediate in the enzymatic formation of germacradienol, germacrene D, and geosmin |
Streptomyces avermitilis |
? |
- |
? |
