EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
4.1.99.14 | (5''R)-alpha-5''(6''H)-bithymine + S-adenosyl-L-methionine |
SPL repairs specifically the 5R isomer. (5''R)-alpha-5''(6''H)-bithymine is the diastereomer produced upon UV irradiation of a TpT dinucleotide |
Clostridium acetobutylicum |
thymidylyl-(3'-5')-thymidylate + 5'-deoxyadenosine + L-methionine |
- |
? |
4.1.99.14 | (5''R)-alpha-5''(6''H)-bithymine + S-adenosyl-L-methionine |
SPL repairs specifically the 5R isomer |
Clostridium acetobutylicum |
thymidylyl-(3'-5')-thymidylate + 5'-deoxyadenosine + L-methionine |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction |
Geobacillus thermodenitrificans |
thymidylyl-(3'-5')-thymidylate |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
- |
Bacillus subtilis |
thymidylyl-(3'->5')-thymidylate |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
i.e. spore photoproduct, an in situ monomerization |
Bacillus subtilis |
thymidylyl-(3'->5')-thymidylate |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
the reaction is highly stereo-selective |
Bacillus subtilis |
thymidylyl-(3'->5')-thymidylate |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
in double-helical DNA |
Geobacillus thermodenitrificans |
thymidylyl-(3'->5')-thymidylate |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
in double-helical DNA |
Clostridium acetobutylicum |
thymidylyl-(3'->5')-thymidylate |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
in double-helical DNA |
Bacillus subtilis |
thymidylyl-(3'->5')-thymidylate |
- |
? |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
- |
Bacillus subtilis 168 |
thymidylyl-(3'->5')-thymidylate |
- |
? |