EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.6.4.B4 | ATP + H2O |
model for the involvement of SsoRal3 in stabilization of the SsoRadA presynaptic filament during strand invasion: SsoRadA presynaptic filament formation and strand invasion in the presence of SsoRal3. SsoRal3 is stored in an inactive state on dsDNA in the absence of ATP. ATP addition permits SsoRal3 binding to ssDNA, stabilizing the presynaptic filament and enhancing strand invasion |
Saccharolobus solfataricus |
ADP + phosphate |
- |
? |
3.6.4.B4 | ATP + H2O |
the enzyme shows almost no ATP hydrolysis in the absence of DNA. SsoRal3 can bind both ss- and dsDNA. The presence of ATP enhances the ability of SsoRal3 to bind ssDNA. The opposite effect is observed when dsDNA was used. When bound to either substrate, the strength of binding is nearly equivalent. SsoRal3 ssDNA binding stabilizes the SsoRadA-ssDNA nucleoprotein complex only when ATP is present |
Saccharolobus solfataricus |
ADP + phosphate |
- |
? |
3.6.4.B4 | ATP + H2O |
model for the involvement of SsoRal3 in stabilization of the SsoRadA presynaptic filament during strand invasion: SsoRadA presynaptic filament formation and strand invasion in the presence of SsoRal3. SsoRal3 is stored in an inactive state on dsDNA in the absence of ATP. ATP addition permits SsoRal3 binding to ssDNA, stabilizing the presynaptic filament and enhancing strand invasion |
Saccharolobus solfataricus P2 |
ADP + phosphate |
- |
? |
3.6.4.B4 | ATP + H2O |
the enzyme shows almost no ATP hydrolysis in the absence of DNA. SsoRal3 can bind both ss- and dsDNA. The presence of ATP enhances the ability of SsoRal3 to bind ssDNA. The opposite effect is observed when dsDNA was used. When bound to either substrate, the strength of binding is nearly equivalent. SsoRal3 ssDNA binding stabilizes the SsoRadA-ssDNA nucleoprotein complex only when ATP is present |
Saccharolobus solfataricus P2 |
ADP + phosphate |
- |
? |