EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.5.4.34 | adenine37 in tRNAAla + H2O |
- |
Drosophila melanogaster |
hypoxanthine37 in tRNAAla + NH3 |
- |
? |
| 3.5.4.34 | adenine37 in tRNAAla + H2O |
- |
Saccharomyces cerevisiae |
hypoxanthine37 in tRNAAla + NH3 |
- |
? |
| 3.5.4.34 | adenine37 in tRNAAla + H2O |
specifically deaminates adenosine 37 to inosine in eukaryotic tRNAAla. This unique modification in tRNAAla is conserved from yeast to man |
Homo sapiens |
hypoxanthine37 in tRNAAla + NH3 |
- |
? |
| 3.5.4.34 | adenine37 in tRNAAla + H2O |
in vitro, the recombinantly expressed and purified hADAT1 protein efficiently and specifically deaminates A37 in the anticodon loop of tRNAAla from higher eukaryotes and with lower efficiency from lower eukaryotes. It does not modify adenosines residing in double-stranded RNA or in pre-mRNAs that serve as substrates for ADAR1 or ADAR2. The anticodon stemloop of tRNAAla alone is not a functional substrate for hADAT1 |
Homo sapiens |
hypoxanthine37 in tRNAAla + NH3 |
- |
? |
| 3.5.4.34 | adenine37 in tRNAAla + H2O |
pre-mRNA and extended dsRNA are not used as substrates by Tad1p in vitro. Tad1p requires both the local conformation of the anticodon loop and a correct folding of the L-shaped tRNAAla substrate to recognize and deaminate A37 |
Saccharomyces cerevisiae |
hypoxanthine37 in tRNAAla + NH3 |
- |
? |
| 3.5.4.34 | adenine37 in tRNAAla + H2O |
the enzyme is specific for adenosine 37 of insect alanine tRNA, it has no activity on dsRNA substrates. Drosophila ADAT1 binds and deaminates tRNAAla from Bombyx mori 100fold more efficiently than the tRNAAla from Saccharomyces cerevisiae |
Drosophila melanogaster |
hypoxanthine37 in tRNAAla + NH3 |
- |
? |
