EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.4.24.B6 | aggrecan + H2O |
cleavage site is Asn341-Phe342 |
Homo sapiens |
? |
- |
? |
| 3.4.24.B6 | ameloblastin + H2O |
MMP-20 cleaves ameloblastin after Pro2, Gln130, Gln139, Arg170, and Ala222 |
Sus scrofa |
ameloblastin fragments |
fragments of 23 kDa, 17 kDa, and 15 kDa |
? |
| 3.4.24.B6 | ameloblastin + H2O |
N-terminal ameloblastin cleavage products of 13, 15, and 17 kDa accumulate in the sheath space throughout the enamel layer |
Sus scrofa |
ameloblastin fragments |
- |
? |
| 3.4.24.B6 | ameloblastin + H2O |
MMP-20 cleaves the recombinant porcine substrate at multiple sites on the N-terminal side of the protein to fragments that show smaller bands of 7-25 kDa and larger products of 45-65 kDa. MMP-20 starts the cleavage on the N-terminal side of the protein, so that the N-terminal cleavage products are smaller than the C-terminal cleavage products digested by MMP-20 initially at one of three sitesafter Gln130. The substrate is digested by MMP-20 initially at one of three sites, after Gln130, Arg170, and Ala222, generating 6 cleavage products. These initial products are then cleaved a second or third time at these same sites, as well as at secondary sites that are located mostly near the C-terminus, after Gly300, Arg319, Ala342, and Asn31 Arg170, and Ala222, generating 6 cleavage products. These initial products are then cleaved a second or third time at these same sites, as well as at secondary sites that are located mostly near the C-terminus, after Gly300, Arg319, Ala342, and Asn31 |
Sus scrofa |
ameloblastin fragments |
mass spectrometry product analysis, overview |
? |
| 3.4.24.B6 | ameloblastin + H2O |
MMP-20 processes ameloblastin during the secretory stage of amelogenesis, cleaves glycosylated recombinant porcine ameloblastin after Pro2, Gln130, Gln139, Arg170, and Ala222. MMP-20 generates the 23-kDa ameloblastin starting at Tyr223, as well as the 17-kDa (Val1-Arg170) and 15-kDa (Val1-Gln130) ameloblastin cleavage products that concentrate in the sheath space during the secretory stage |
Sus scrofa |
? |
- |
? |
| 3.4.24.B6 | ameloblastin + H2O |
cleaves glycosylated recombinant porcine ameloblastin after Pro2, Gln130, Gln139, Arg170, and Ala222. MMP-20 generates the 23-kDa ameloblastin starting at Tyr223, as well as the 17-kDa (Val1-Arg170) and 15-kDa (Val1-Gln130) ameloblastin cleavage products that concentrate in the sheath space during the secretory stage |
Sus scrofa |
? |
- |
? |
| 3.4.24.B6 | amelogenin + H2O |
- |
Sus scrofa |
amelogenin fragments |
- |
? |
| 3.4.24.B6 | amelogenin + H2O |
Mmp-20 cleaves amelogenin sequences after Pro162, Ser148, His62, Ala63, and Trp45. These cleavages generate all of the major cleavage products from tyrosine-rich amelogenin polypeptide that accumulate in porcine secretory stage enamel, i.e. the 23 kDa, 20 kDa, 13 kDa, 11 kDa, and 6 kDa amelogenins. And Mmp-20 cleaves leucine rich-amelogenin protein after Pro45 and Pro40, producing the two LRAP products, Mmp-20 cleavage site preferences, overview |
Sus scrofa |
amelogenin fragments |
- |
? |
| 3.4.24.B6 | amelogenin + H2O |
cleaves amelogenin between Trp45 and Leu46 |
Homo sapiens |
23 kDa amelogenin + 5 kDa tyrosine-rich amelogenin peptide |
- |
? |
| 3.4.24.B6 | amelogenin + H2O |
- |
Mus musculus |
? |
- |
? |
