EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.4.22.2 | (RS)-mandelic hydrazide + benzyloxycarbonyl-Ala |
- |
Carica papaya |
N1-(benzyloxycarbonyl-Ala)-N2-[(R)-mandelyl]hydrazine + N1-(benzyloxycarbonyl-Ala)-N2-[(S)-mandelyl]hydrazine |
mixture of diastereoisomers containing 73% N1-(benzyloxycarbonyl-Ala)-N2-[(R)-mandelyl]hydrazine |
? |
| 3.4.22.2 | (RS)-mandelic hydrazide + benzyloxycarbonyl-Gly |
- |
Carica papaya |
N1-(benzyloxycarbonyl-Gly)-N2-[(R)-mandelyl]hydrazine + N1-(benzyloxycarbonyl-Gly)-N2-[(S)-mandelyl]hydrazine |
- |
? |
| 3.4.22.2 | (RS)-mandelic hydrazide + hippuric acid |
- |
Carica papaya |
? |
- |
? |
| 3.4.22.2 | (RS)-mandelic hydrazide + N(tert-amyloxycarbonyl)-Gly |
- |
Carica papaya |
(+)-N1-(tert-amyloxycarbonyl-Gly)-NH2-[(R)-mandelyl]hydrazine + N1-(tert-butoxycarbonyl-Gly)-N2-[(S)-mandelyl]hydrazine |
- |
? |
| 3.4.22.2 | (RS)-mandelic hydrazide + N-(tert-butyloxycarbonyl)-Gly |
- |
Carica papaya |
(+)-N1-(tert-butyloxycarbonyl-Gly)-N2[(R)-mandelyl]hydrazine + (+)-(N1)-(tert-butyloxycarbonyl-Gly)-N2[(S)-mandelyl]hydrazine |
- |
? |
| 3.4.22.2 | 2-(amino)ethyl 2'-pyridyl disulfide + H2O |
- |
Carica papaya |
? |
- |
? |
| 3.4.22.2 | 3-(amino)trimethylene 2'-pyridyl disulfide + H2O |
- |
Carica papaya |
? |
- |
? |
| 3.4.22.2 | 4-(amino)-tetramethylene 2'-pyridyl disulfide + H2O |
- |
Carica papaya |
? |
- |
? |
| 3.4.22.2 | Ac-L-Phe-Gly 4-nitroanilide + H2O |
whole hydrolysis process includes two stages: acylation and deacylation. The first step is a proton transfer to form a zwitterionic form (i.e. Cys-S-/His-H+ion-pair), and the second step is the nucleophilic attack on the carboxyl carbon of the substrate accompanied with the dissociation of 4-nitroaniline. The deacylation stage includes the nucleophilic attack of a water molecule on the carboxyl carbon of the substrate and dissociation between the carboxyl carbon of the substrate and the sulfhydryl sulfur of Cys25 side chain. The acylation is rate-limiting |
Carica papaya |
Ac-L-Phe-Gly + 4-nitroaniline |
- |
? |
| 3.4.22.2 | acetyl-Ala-Ala-Ala-p-nitroanilide + H2O |
- |
Carica papaya |
? |
- |
? |
