EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.2.1.B40 | 2-nitrophenyl beta-D-glucopyranoside + H2O |
- |
Pyrococcus horikoshii |
2-nitrophenol + beta-D-glucopyranose |
- |
? |
3.2.1.B40 | 2-nitrophenyl beta-D-glucopyranoside + H2O |
wide substrate specificity belonging to family 1 of glycoside hydrolases classification. The mutant enzyme E324G does not have any activity with the substrate 2-nitrophenyl-beta-D-glucopyranose. However, in the presence of 3 M sodium formate the mutant is reactivated |
Pyrococcus horikoshii |
2-nitrophenol + beta-D-glucopyranose |
- |
? |
3.2.1.B40 | 2-nitrophenyl beta-D-glucopyranoside + H2O |
- |
Pyrococcus horikoshii OT-3 |
2-nitrophenol + beta-D-glucopyranose |
- |
? |
3.2.1.B40 | 2-nitrophenyl beta-D-glucopyranoside + H2O |
wide substrate specificity belonging to family 1 of glycoside hydrolases classification. The mutant enzyme E324G does not have any activity with the substrate 2-nitrophenyl-beta-D-glucopyranose. However, in the presence of 3 M sodium formate the mutant is reactivated |
Pyrococcus horikoshii OT-3 |
2-nitrophenol + beta-D-glucopyranose |
- |
? |
3.2.1.B40 | 4-nitrophenyl beta-D-galactopyranoside + H2O |
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside |
Pyrococcus horikoshii |
4-nitrophenol + D-galactose |
- |
? |
3.2.1.B40 | 4-nitrophenyl beta-D-galactopyranoside + H2O |
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside |
Pyrococcus horikoshii OT-3 |
4-nitrophenol + D-galactose |
- |
? |
3.2.1.B40 | 4-nitrophenyl beta-D-glucopyranoside + H2O |
kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside |
Pyrococcus horikoshii |
4-nitrophenol + D-glucose |
- |
? |
3.2.1.B40 | 4-nitrophenyl beta-D-glucopyranoside + H2O |
kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside |
Pyrococcus horikoshii OT-3 |
4-nitrophenol + D-glucose |
- |
? |
3.2.1.B40 | 4-nitrophenyl beta-D-mannopyranoside + H2O |
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside |
Pyrococcus horikoshii |
4-nitrophenol + D-mannose |
- |
? |
3.2.1.B40 | 4-nitrophenyl beta-D-xylopyranoside + H2O |
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside |
Pyrococcus horikoshii |
4-nitrophenol + D-xylose |
- |
? |