EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.2.1.179 | beta-D-4-deoxy-DELTA-4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O |
enzyme activity is measured by monitoring the decrease in absorbance at 235 nm, corresponding to the loss of the C=C double bond of the substrate because the pyranose ring of the released DELTAGlcA readily opens so that it is nonenzymatically converted to alpha-keto acid through the loss of the double bond |
Bacillus sp. (in: Bacteria) |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA-4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O |
the enzyme strongly recognizes DELTA-4,5-GlcA at subsite -1 through the formation of hydrogen bonds and stacking interactions, and prefers N-acetyl-D-galactosamine and glucose rather than N-acetyl-D-glucosamine as a residue accommodated in subsite +1, due to the steric hindrance |
Bacillus sp. (in: Bacteria) |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA-4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O |
the enzyme strongly recognizes DELTA-4,5-GlcA at subsite -1 through the formation of hydrogen bonds and stacking interactions, and prefers N-acetyl-D-galactosamine and glucose rather than N-acetyl-D-glucosamine as a residue accommodated in subsite +1, due to the steric hindrance |
Bacillus sp. (in: Bacteria) GL1 |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA-4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O |
enzyme activity is measured by monitoring the decrease in absorbance at 235 nm, corresponding to the loss of the C=C double bond of the substrate because the pyranose ring of the released DELTAGlcA readily opens so that it is nonenzymatically converted to alpha-keto acid through the loss of the double bond |
Bacillus sp. (in: Bacteria) GL1 |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O |
114% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
Bacillus sp. (in: Bacteria) |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O |
114% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
Bacillus sp. (in: Bacteria) GL1 |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O |
- |
Bacillus sp. (in: Bacteria) |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O |
- |
Bacillus sp. (in: Bacteria) GL1 |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O |
25.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
Bacillus sp. (in: Bacteria) |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc6S |
- |
? |
3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O |
25.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp |
Bacillus sp. (in: Bacteria) GL1 |
4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc6S |
- |
? |