EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.2.1.157 | iota-/nu-carrageenan + H2O |
- |
Alteromonas fortis |
hydrolyzed iota/nu-carrageenan |
the smallest hybrid is an octasaccharide with a iota-iota-nu-iota structure, the second fraction is composed of two decasaccharides with iota-iota-iota-nu-iota and iota-[iota/nu]iota-iota structures, the third fraction is a mixture of dodecasaccharides which contains at least a iota-iota-iota-iota-nu-iota oligosaccharide |
? |
3.2.1.157 | iota-carrageenan + H2O |
- |
Alteromonas fortis |
hydrolyzed iota-carrageenan |
- |
? |
3.2.1.157 | iota-carrageenan + H2O |
from an open conformation which allows the initial endo-attack of iota-carrageenan chains, the enzyme switches to a closed-tunnel form, consistent with its highly processive character |
Alteromonas fortis |
hydrolyzed iota-carrageenan |
- |
? |
3.2.1.157 | iota-carrageenan + H2O |
hybrid kappa/iota-carrageeans extracted from Gigartina skottsbergii, Chondracanthus chamissoi, and Chondrus crispus are incubated with Pseudoalteromonas carrageenovora kappa-carrageenase and Alteromonas fortis iota-carrageenase. A low percentage of degradation is observed after treatment by iota-carrageenase, suggesting that long segments of iota-carrabiose or block of iota-carrageenan are low in abundance |
Alteromonas fortis |
hydrolyzed iota-carrageenan |
- |
? |
3.2.1.157 | iota-carrageenan + H2O |
- |
Alteromonas fortis |
iota-neocarratetraose sulfate + iota-neocarrahexaose sulfate |
- |
? |
3.2.1.157 | iota-carrageenan + H2O |
endohydrolytic cleavage, the enzyme proceeds with an overall inversion of the anomeric configuration |
Zobellia galactanivorans |
iota-neocarratetraose sulfate + iota-neocarrahexaose sulfate |
major end products |
? |
3.2.1.157 | iota-carrageenan + H2O |
the enzyme cleaves beta-1,4 linkages in iota-carrageenan to produce a high ratio of iota-carrageenan tetramer, more than 75% of the total product |
Microbulbifer thermotolerans |
iota-neocarratetraose sulfate + iota-neocarrahexaose sulfate |
- |
? |
3.2.1.157 | iota-carrageenan + H2O |
the enzyme cleaves beta-1,4 linkages in iota-carrageenan to produce a high ratio of iota-carrageenan tetramer, more than 75% of the total product |
Microbulbifer thermotolerans JAMB-A94T |
iota-neocarratetraose sulfate + iota-neocarrahexaose sulfate |
- |
? |
3.2.1.157 | iota-carrageenan + H2O |
- |
Alteromonas fortis |
neo-iota-carratetraose + neo-iota-carrahexaose |
most abundant products |
? |
3.2.1.157 | iota-carrageenan + H2O |
- |
uncultured bacterium |
? |
- |
? |