EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
    2.8.4.1 | 2-(methylthio)ethanesulfonate + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate |
i.e. CoM and CoB |
Methanothermobacter marburgensis |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | 2-(methylthio)ethanesulfonate + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate |
i.e. CoM and CoB, the enzyme exists in the the inactive Ni(II) MCRox1-silent form and the active Ni(I) MCRred1 form with transition from MCRred1 to MCRred2 forms, the protein is able to undergo a conformational change upon binding of the second substrate. Analysis of the catalytic mechanism of the reduction at the nickel center using inhibitory fluorescent trifluoromethyl thio esters of the substrate CoB for spectroscopic analysis of the structure of the enzyme-cofactor complex, derivatives synthesis, overview |
Methanothermobacter marburgensis |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | 2-(methylthio)ethansulfonate + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate |
i.e. CoM and CoB |
Methanothermobacter marburgensis |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | CH3-S-CoM + HS-CoB |
- |
Methanothermobacter marburgensis |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | CH3-S-CoM + HS-CoB |
- |
uncultured archaeon |
CoM-S-S-CoB + methane |
- |
r |
| 2.8.4.1 | CH3-S-CoM + HS-CoB |
i.e. methyl-coenzyme M + coenzyme B |
Methanothermobacter marburgensis |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | CH3-S-CoM + HS-CoB |
key step in the convertion of C1 substrates or acetate to methane thereby providing energy for the cell |
Methanothermobacter marburgensis |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | CH3-S-CoM + HS-CoB |
MCR catalyzes the first proposed step in anaerobic methane oxidation and terminal step in methanogenesis by using N-7-mercaptoheptanolyl-threonine phosphate, i.e. CoB-SH. as the two-electron donor to reduce 2-(methylthiol)-ethane sulfonate, i.e. methyl-SCoM, to methane, and producing the heterodisulfide, CoBS-SCoM |
Methanothermobacter marburgensis |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | CH3-S-CoM + HS-CoB |
MCR catalyzes the methane-forming step in methanogenic archaea |
Methanosarcina barkeri |
CoM-S-S-CoB + methane |
- |
? |
| 2.8.4.1 | CH3-S-CoM + HS-CoB |
MCR catalyzes the methane-forming step in methanogenic archaea |
Methanocaldococcus jannaschii |
CoM-S-S-CoB + methane |
- |
? |
