EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.7.7.87 | L-threonine + ATP + bicarbonate |
- |
Escherichia coli |
L-threonylcarbamoyladenylate + diphosphate + H2O |
threonylcarbamoyladenosine i.e. t6A |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
results indicate that the universally conserved SUA5 family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine |
Saccharomyces cerevisiae |
L-threonylcarbamoyladenylate + diphosphate + H2O |
threonylcarbamoyladenosine i.e. t6A |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
results indicate that the universally conserved YrdC family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine |
Escherichia coli |
L-threonylcarbamoyladenylate + diphosphate + H2O |
threonylcarbamoyladenosine i.e. t6A |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
determination of thermodynamic parameters of L-threonine, L-serine, ATP, ADP, AMP binding to Sua5 by ITC measurements |
Sulfurisphaera tokodaii |
L-threonylcarbamoyladenylate + diphosphate + H2O |
- |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
YrdC is an ATPase that binds L-threonine and tRNAs that respond to codons beginning with adenosine. The enzyme recognizes the heptadecamer anticodon stem and loop of tRNALysUUU |
Escherichia coli |
L-threonylcarbamoyladenylate + diphosphate + H2O |
- |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
beside YrdC, proteins YgjD, YjeE and YeaZ are necessary and sufficient for t6A biosynthesis in vitro |
Escherichia coli |
L-threonylcarbamoyladenylate + diphosphate + H2O |
L-threonylcarbamoyladenylate i.e. t6A |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
PaSua5 and PaKEOPS together catalyse the biosynthesis of L-threonylcarbamoyladenylate i.e. t6A |
Pyrococcus abyssi |
L-threonylcarbamoyladenylate + diphosphate + H2O |
- |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
ScSua5 and ScKEOPS together catalyse the biosynthesis of L-threonylcarbamoyladenylate i.e. t6A |
Saccharomyces cerevisiae |
L-threonylcarbamoyladenylate + diphosphate + H2O |
- |
? |
2.7.7.87 | L-threonine + ATP + bicarbonate |
the combination of Qri7 and Sua5 is necessary and sufficient for L-threonylcarbamoyladenylate biosynthesis |
Saccharomyces cerevisiae |
L-threonylcarbamoyladenylate + diphosphate + H2O |
- |
? |
2.7.7.87 | L-threonine + ATP + CO2 |
catalyzed by YwlC(TsaC) |
Bacillus subtilis |
L-threonylcarbamoyl-AMP + diphosphate |
L-threonylcarbamoyl-AMP i.e. TC-AMP, isolated by HPLC and characterized by mass spectrometry and 1H-NMR |
? |