EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.6.1.98 | more |
2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid WbpE product identification by mass spectrometry, overview |
Pseudomonas aeruginosa |
? |
- |
? |
2.6.1.98 | more |
WbpB and WbpE are a dehydrogenase/aminotransferase pair that converts UDP-N-acetyl-D-glucosaminuronate, UDP-GlcNAcA, to UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate, UDP-GlcNAc(3NH2)A, in a coupled reaction via a unique NAD+ recycling pathway, large-scale coupled reaction WpbB/WpbE, overview. WbpA, WbpB, WbpE, WbpD and WbpI can be combined in vitro to generate UDP-ManNAc(3NAc)A in a single reaction vessel. Addition of 0.2 mM NAD+, for WbpB, and 0.1 mM pyridoxal 5'-phosphate, for WbpE, to the reaction mixture aids in achieving complete turnover of substrate, which implies that the heterologously expressed proteins are not saturated with cofactor due to the limiting intracellular levels of both NAD+ and pyridoxal 5'-phosphate in Escherichia coli. WbpB/WbpE substrate specificity, overview |
Pseudomonas aeruginosa |
? |
- |
? |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
- |
Pseudomonas aeruginosa |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
r |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
- |
Pseudomonas aeruginosa |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
? |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
transaminase WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate |
Pseudomonas aeruginosa |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
r |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate |
Pseudomonas aeruginosa |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
r |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate |
Bordetella pertussis |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
r |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
transaminase WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate. Cofactor pyridoxal 5'-phosphate is converted to pyridoxamine phosphate during the reaction |
Pseudomonas aeruginosa |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
r |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid |
Pseudomonas aeruginosa |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
r |
2.6.1.98 | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate |
- |
Pseudomonas aeruginosa ATCC 15692 |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate |
- |
? |