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Results 1 - 6 of 6
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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the reaction diagram Show all sequences 2.4.1.291more in vitro transfer of N,N'-diacetylbacillosamine phosphate onto Und-P and the in vitro enzymatic synthesis of the heptasacccharide using a chemically synthesized undecaprenyl diphosphate-linked N,N'-diacetylbacillosamine. The reactions of PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel, NMR spectrometric product analysis Campylobacter jejuni ? - ?
Display the reaction diagram Show all sequences 2.4.1.291more with polyisoprenols substrates of varying length, the enzyme exhibits specificity with respect to cis-double bond geometry and alpha-unsaturation of the native substrate are important features, while the precise polyisoprene length may be less critical. PglJ accepts the prenol-9 substrate with efficiencies equal to that of the native undecaprenol-based substrate Campylobacter jejuni ? - ?
Display the reaction diagram Show all sequences 2.4.1.291UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - Campylobacter jejuni UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - ?
Display the reaction diagram Show all sequences 2.4.1.291UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol the sugar 2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose, termed N,N'-diacetylbacillosamine, i.e Bac2,4diNAc, is the first carbohydrate in the glycoprotein N-linked heptasaccharide. In vitro biosynthesis of the complete heptasaccharide lipid-linked donor by coupling the action of eight enzymes, PglF, PglE, PglD, PglC, PglA, PglJ, PglH, and PglI, in the Pgl pathway in a single reaction vessel, overview Campylobacter jejuni UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - ?
Display the reaction diagram Show all sequences 2.4.1.291UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol in the assay, radiolabeled UDP-GalNAc is used with radiolabeled polyisoprenyl diphosphate Bac2,4diNAc-[3H]GalNAc, enzymatically synthesized Campylobacter jejuni UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - ?
Display the reaction diagram Show all sequences 2.4.1.291UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - Campylobacter jejuni NCTC 11168 UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - ?
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