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Results 1 - 6 of 6
EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the reaction diagram Show all sequences 2.4.1.290more starting from UDP-GalNAc, UDP-Glc, Und-P and chemically synthesized UDP-Bac-2,4-NAc, the reactions of recombinant PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel to synthesize the bacterial glycan heptasaccharide, overview. Presence of GST-PglF, PglE and PglD in the same reaction vessel does not inhibit the downstream reactions. In N-linked glycoproteins the heptasaccharide is covalently attached at the N,N'-diacetylbacillosamine through a beta-linkage to the amide nitrogen of an asparagine side chain in the canonical sequence Asn-Xaa-Ser/Thr motif, where Xaa can be any amino acid except proline Campylobacter jejuni ? - ?
Display the reaction diagram Show all sequences 2.4.1.290more development and optimization of an assay method to monitor the transfer of GalNAc from the hydrophilic UDP-linked carrier to the lipophilic UndPP-diNAcBac, i.e. 2,4-diacetamido-2,4,6-trideoxyglucose Campylobacter jejuni ? - ?
Display the reaction diagram Show all sequences 2.4.1.290more starting from UDP-GalNAc, UDP-Glc, Und-P and chemically synthesized UDP-Bac-2,4-NAc, the reactions of recombinant PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel to synthesize the bacterial glycan heptasaccharide, overview. Presence of GST-PglF, PglE and PglD in the same reaction vessel does not inhibit the downstream reactions. In N-linked glycoproteins the heptasaccharide is covalently attached at the N,N'-diacetylbacillosamine through a beta-linkage to the amide nitrogen of an asparagine side chain in the canonical sequence Asn-Xaa-Ser/Thr motif, where Xaa can be any amino acid except proline Campylobacter jejuni NCTC 11168 ? - ?
Display the reaction diagram Show all sequences 2.4.1.290UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - Campylobacter jejuni UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - ?
Display the reaction diagram Show all sequences 2.4.1.290UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol one N-acetylgalactosamine is linked to the polyisoprenepyrophosphate-bound N,N'-diacetylbacillosamine by Cj1125c (PglA) to form GalNAc-alpha1,3-Bac2,4diNAc-alpha1-PP-Und Campylobacter jejuni UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - ?
Display the reaction diagram Show all sequences 2.4.1.290UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - Campylobacter jejuni NCTC 11168 UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol - ?
Results 1 - 6 of 6