EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.250 | 1D-myo-inositol 3-phosphate + UDP-N-acetyl-alpha-D-glucosamine |
the reaction proceeds via a front-side SNi-like concerted reaction mechanism |
Corynebacterium glutamicum |
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside 3-phosphate + UDP |
- |
? |
2.4.1.250 | 1D-myo-inositol 3-phosphate + UDP-N-acetyl-alpha-D-glucosamine |
the reaction proceeds via a front-side SNi-like concerted reaction mechanism |
Corynebacterium glutamicum ATCC 13032 |
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside 3-phosphate + UDP |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 1-phosphate |
at 10% of the activity with 1D-myo-inositol 3-phosphate |
Mycolicibacterium smegmatis |
? |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 1-phosphate |
at 10% of the activity with 1D-myo-inositol 3-phosphate |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 |
? |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate |
1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, committed step of mycothiol biosynthesis |
Corynebacterium glutamicum |
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate |
1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis |
Mycolicibacterium smegmatis |
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate |
1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis |
Mycobacterium tuberculosis |
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate |
1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate |
Mycobacterium tuberculosis |
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate |
1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Little activity is obtained with myo-inositol, 1D-myo-inositol 1-phosphate, or myo-inositol 2-phosphate as the N-acetylglucosamine acceptor. 1D-myo-inositol 1-phosphate is neither a good substrate nor an inhibitor of MshA. Therfore the commercially available 1DL-myo-inositol 1-phosphate can be used as substrate for MshA assays without complications |
Mycolicibacterium smegmatis |
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP |
- |
? |
2.4.1.250 | UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate |
1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Molecular models of the ternary complex suggest a mechanism in which the beta-phosphate of the substrate, UDP-N-acetylglucosamine, promotes the nucleophilic attack of the 3-hydroxyl group of 1D-myo-inositol 3-phosphate while at the same time promoting the cleavage of the sugar nucleotide bond |
Corynebacterium glutamicum |
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP |
- |
? |