EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.185 | more |
does not use UDP-glucuronic acid, UDP-galactose, ADP-glucose, GDP-glucose, and TDP-glucose with esculetin as the glycosyl acceptor |
Lycium barbarum |
? |
- |
? |
2.4.1.185 | more |
the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo |
Citrus sinensis |
? |
- |
- |
2.4.1.185 | more |
in vitro, enzyme CsUGT76F1 can function as flavonoid 3-O-, 7-O-, and 3,7-O-glucosyltransferase, and as 7-O-rhamnosyltransferase with a broad range of flavonoid substrates that can be glucosylated at their 3- and/or 7-hydrogen sites by the recombinant enzyme, including flavanones, flavonols, and flavons, e.g. hesperetin, naringenin, diosmetin, quercetin, and kaempferol as acceptor substrates, and UDP-glucose and UDP-rhamnose as the sugar donors. Mass spectrometric product analysis (UPLC-Q-TOF-MS). The recombinant UGT can only accept UDP-rhamnose to rhamnosylate the 7-hydrogen position of quercetin and kaempferol, but not to catalyze the rhamnosylation of hesperetin, naringenin, and diosmetin. No flavonoid O-rutinoside (rhamnosyl-alpha-1,6-glucose) or O-neohesperidose (rhamnosyl-alpha-1,2-glucose) products are detected |
Citrus sinensis |
? |
- |
- |
2.4.1.185 | UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone |
- |
Citrus maxima |
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside |
- |
? |
2.4.1.185 | UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone |
- |
Citrus mitis |
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside |
- |
? |
2.4.1.185 | UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone |
enzyme does not accept other flavanone or flavonol aglycones |
Citrus x paradisi |
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside |
trivial name prunin |
? |
2.4.1.185 | UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone |
peak I-III |
Petunia x hybrida |
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside |
- |
? |
2.4.1.185 | UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone |
trivial name naringenin |
Citrus x paradisi |
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside |
- |
? |
2.4.1.185 | UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone |
trivial name naringenin |
Citrus x paradisi |
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside |
trivial name prunin |
? |
2.4.1.185 | UDP-alpha-D-glucose + apigenin |
peak I-III, 49%, 45% and 95% of activity with naringenin respectively |
Petunia x hybrida |
UDP + apigenin 7-O-beta-D-glucoside |
- |
? |