EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.3.2.27 | 2 [gp78-ubiquitin-carrier protein Ub2g2]-S-ubiquitinyl-L-cysteine |
use of mouse Ube2g2 ubiquitin conjugating enzyme. Ube2g2/gp78-mediated polyubiquitination involves preassembly of Lys 48-linked ubiquitin chains at the catalytic cysteine residue C89 of Ube2g2. The growth of Ube2g2-anchored ubiquitin chains seems to be mediated by an aminolysis-based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in its active site. Polyubiquitination of a substrate can be achieved by transferring preassembled ubiquitin chains from Ube2g2 to a lysine residue in a substrate |
Homo sapiens |
[gp78-ubiquitin-carrier protein Ub2g2]-S-[ubiquitinyl-N6-ubiquitinyl-L-lysine46]-L-cysteine |
- |
? |
| 2.3.2.27 | more |
degradation of the cytoplasmic misfolded protein DELTAssCL*myc, a derivative of signal sequence delted mutated carboxypeptidase yscY |
Saccharomyces cerevisiae |
? |
- |
? |
| 2.3.2.27 | more |
ubiquitination activity of the fusion protein CaRING1-maltose-binding protein MBP are not displayed in the absence of E1, E2, E3, ATP or ubiquitin |
Capsicum annuum |
? |
ubiquitination activity of the fusion protein CaRING1-maltose-binding protein MBP is detected using anti-ubiquitin and anti-MBP antibody |
? |
| 2.3.2.27 | more |
isoform ACRE276 is an E3 ubiquitin ligase requiring an intact U-box domain |
Solanum lycopersicum |
? |
- |
? |
| 2.3.2.27 | more |
isoform ACRE276 is an E3 ubiquitin ligase requiring an intact U-box domain |
Nicotiana tabacum |
? |
- |
? |
| 2.3.2.27 | more |
isoform ACRE276 is an E3 ubiquitin ligase requiring an intact U-box domain |
Arabidopsis thaliana |
? |
- |
? |
| 2.3.2.27 | more |
isoform GRN1 interacts with melanocortin receptor and decreases melanocortin receptors MC1R and MC4R signaling to cAMP. Inhibition of MC1R signaling by MGRN1 is not dependent on receptor down-regulation as a result of internalization or degradation. Receptor ubiquitylation is not a requisite for inhibition of signaling |
Homo sapiens |
? |
- |
? |
| 2.3.2.27 | more |
isoform RMA2 exhibits a high degree of E3 activity with E2 enzyme UBC8 and a moderate level with E2 enzyme UBC10 and no activity wih E2 enzymes UBC5 and UBC13 |
Arabidopsis thaliana |
? |
- |
? |
| 2.3.2.27 | more |
isoform RN181 interacts with the KVGFFKR region of platelet integrin alphaIIbbeta3. The integrin is not an endogenous substrate for RN181-directed ubiquitination |
Homo sapiens |
? |
- |
? |
| 2.3.2.27 | more |
isoform RNF43 interacts with HAP95, a chromatin-associated protein interfacing the nuclear envelope. HAP95 is ubiquitinated and subjected to a proteasome-dependent degradation pathway, however, HAP95 is unlikely to serve as a substrate of RNF43 ubiquitin ligase |
Homo sapiens |
? |
- |
? |
