EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.3.2.20 | L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe |
- |
Streptomyces noursei |
tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) |
- |
? |
2.3.2.20 | L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe |
- |
Streptomyces noursei |
tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) |
intermediate product of the biosynthetic pathway to albonoursin, cyclo(alpha,beta-dehydroPhe-alpha,beta-dehydroLeu) |
? |
2.3.2.20 | L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe |
highest activity |
Streptomyces albulus |
tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) |
- |
? |
2.3.2.20 | L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe |
second best activity |
Bacillus sp. NSP9.1 |
tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) |
- |
? |
2.3.2.20 | L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe |
second best activity |
Bacillus sp. 171095_106 |
tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) |
- |
? |
2.3.2.20 | L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe |
the enzyme uses more efficiently Phe-tRNAPhe than any of the Leu-tRNALeu. The efficiency of cyclo(L-phenylalanyl-L-phenylalanyl) synthesis is not affected by the presence of Leu-tRNALeuGAG or Leu-tRNALeuTAA indicating that these molecules do not compete with Phe-tRNAPhe for binding to the enzyme. In contrast, Leu-tRNALeuCAA, Leu-tRNALeuTAG or or Leu-tRNALeuCAG isoacceptors inhibit cyclo(L-phenylalanyl-L-phenylalanyl) synthesis revealing a competition with Phe-tRNAPhe |
Streptomyces noursei |
tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) |
- |
? |
2.3.2.20 | L-phenylalanyl-tRNAPhe + L-leucyl-tRNALeu |
- |
Streptomyces noursei |
tRNAPhe + tRNALeu + cyclo(L-phenylalanyl-L-leucyl) |
- |
? |
2.3.2.20 | L-phenylalanyl-tRNAPhe + L-methionyl-tRNAMet |
lowest activity |
Streptomyces albulus |
tRNAPhe + tRNAMet + cyclo(L-phenylalanyl-L-methionyl) |
- |
? |
2.3.2.20 | L-phenylalanyl-tRNAPhe + L-methionyl-tRNAMet |
lowest activity |
Bacillus sp. NSP9.1 |
tRNAPhe + tRNAMet + cyclo(L-phenylalanyl-L-methionyl) |
- |
? |
2.3.2.20 | L-phenylalanyl-tRNAPhe + L-methionyl-tRNAMet |
lowest activity |
Bacillus sp. 171095_106 |
tRNAPhe + tRNAMet + cyclo(L-phenylalanyl-L-methionyl) |
- |
? |