EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.3.1.236 | acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ |
- |
Streptomyces sahachiroi |
5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ |
- |
? |
2.3.1.236 | acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ |
- |
Streptomyces sahachiroi |
5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ |
incubation of substrates with isoform AziB alone leads to a truncated single-ring product, 2-methylbenzoic acid. Presence of thioesterase AziG acting as a chain elongation and cyclization domain is required for additional two rounds of chain extension to form the expected product, 5-methyl-1-naphthoate. None of the other thioesterase domains (AziA6, AziA7, AziA8) are capable of catalyzing the reaction |
? |
2.3.1.236 | acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ |
the multi-domain polyketide synthase involved in the synthesis of azinomycin B |
Streptomyces sahachiroi |
5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ |
- |
? |
2.3.1.236 | acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ |
- |
Streptomyces sahachiroi NRRL 2485 |
5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ |
incubation of substrates with isoform AziB alone leads to a truncated single-ring product, 2-methylbenzoic acid. Presence of thioesterase AziG acting as a chain elongation and cyclization domain is required for additional two rounds of chain extension to form the expected product, 5-methyl-1-naphthoate. None of the other thioesterase domains (AziA6, AziA7, AziA8) are capable of catalyzing the reaction |
? |