EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.1.1.361 | 3 S-adenosyl-L-methionine + a [histone H4]-L-lysine20 |
- |
Homo sapiens |
3 S-adenosyl-L-homocysteine + a [histone H4]-N6,N6,N6-trimethyl-L-lysine20 |
- |
? |
| 2.1.1.361 | more |
In addition to K20 (the target methylation site), R17 and H18 are very important specificity determinants for SET8 for substrate recognition. Any other amino acid introduced at these positions completely abolishes the activity of SET8. SET8 equally accepts K and R at position 19, followed by Y and other hydrophobic amino acids like L, H and I. Residues on the C-terminal side of K20 also play important roles in the specificity of SET8. The enzyme accepts majorly I at the -1 position followed by V. Other hydrophobic amino acids like F, Y and L give weaker methylation signals. SET8 also exhibits strong specificity on L22 and R23. At L22 some other hydrophobic amino acids are tolerated. At the +3 position, SET8 majorly prefers R, however it exhibits weak methylation when itis exchanged with other amino acids. SET8 activity is strongly reduced by the arginine methylation at positions R17 in case of H4 and R379 in case of p53 |
Homo sapiens |
? |
- |
? |
| 2.1.1.361 | more |
enzyme binding to the nucleosome protects it from degradation during the cell cycle. Full length enzyme has 3.8% of its histone methyltransferase activity on nucleosomes containing the H2A/E61A/ E64A/D90A/E92A mutations compared to wild type nucleosomes |
Homo sapiens |
? |
- |
? |
| 2.1.1.361 | more |
the enzyme is completely inactive toward histone H4 with a K20L mutation |
Homo sapiens |
? |
- |
? |
| 2.1.1.361 | more |
the enzyme is completely inactive toward histone H4 with a K20L mutation |
Drosophila melanogaster |
? |
- |
? |
| 2.1.1.361 | more |
the proteolytic degradation of the enzyme is controlled by the SCFSlimb ligase complex |
Drosophila melanogaster |
? |
- |
? |
| 2.1.1.361 | more |
no methylation of Numb protein by the enzyme is detectable |
Homo sapiens |
? |
- |
? |
| 2.1.1.361 | more |
the enzyme interacts with the UBC9 E2 SUMO conjugating enzyme and is preferentially modified with SUMO1 in vivo |
Homo sapiens |
? |
- |
? |
| 2.1.1.361 | S-adenosyl-L-methionine + a [histone H4]-L-lysine20 |
- |
Drosophila melanogaster |
S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20 |
- |
? |
| 2.1.1.361 | S-adenosyl-L-methionine + a [histone H4]-L-lysine20 |
- |
Homo sapiens |
S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20 |
- |
? |
