EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.292 | more |
the purified enzyme does not catalyse methylation of the aglycones carminomycinone or 13-dihydrocarminomycinone |
Streptomyces sp. |
? |
- |
? |
2.1.1.292 | more |
the enzyme possesses rather relaxed substrate specificity in regard to modifications in the polyaromatic anthracycline ring system, but it is quite specific with respect to the length of the carbohydrate chain at C-7, accepting only monoglycosides. In addition, DnrK has even been shown to be able to methylate various flavonoids. Discovery of a 10-decarboxylation activity of DnrK |
Streptomyces peucetius |
? |
- |
- |
2.1.1.292 | more |
the enzyme has been reported to methylate various modified anthracyclines and flavonoids as surrogate substrates. 2-Chloro-4-nitrophenol is a substrate of DnrK. High-throughput DnrK colorimetric assay with the surrogate acceptor 2-chloro-4-nitrophenol and the different cosubstrates. No activity with derivative S-(7-deazaadenosyl)-[(3S)-3-amino-3-(1H-tetrazol-5-yl)propyl](methyl)sulfanium |
Streptomyces peucetius |
? |
- |
- |
2.1.1.292 | S-adenosyl-L-methionine + 10-carboxy-13-deoxycarminomycin |
- |
Streptomyces sp. |
S-adenosyl-L-homocysteine + 10-carboxy-13-deoxydaunorubicin |
- |
? |
2.1.1.292 | S-adenosyl-L-methionine + 11-deoxy-beta-rhodomycin T |
- |
Streptomyces peucetius |
S-adenosyl-L-homocysteine + 4-O-methyl-11-deoxy-beta-rhodomycin T |
- |
? |
2.1.1.292 | S-adenosyl-L-methionine + 13-deoxycarminomycin |
- |
Streptomyces sp. |
S-adenosyl-L-homocysteine + 13-deoxydaunorubicin |
- |
? |
2.1.1.292 | S-adenosyl-L-methionine + 13-dihydrocarminomycin |
- |
Streptomyces sp. |
S-adenosyl-L-homocysteine + daunorubicinol |
- |
? |
2.1.1.292 | S-adenosyl-L-methionine + 13-dihydrocarminomycin |
terminal enzyme in the daunomycin biosynthesis pathway |
Streptomyces sp. |
S-adenosyl-L-homocysteine + daunorubicinol |
- |
? |
2.1.1.292 | S-adenosyl-L-methionine + 13-dihydrocarminomycin |
the enzyme shows a slightly higher affinity for carminomycin than for 13-dihydrocarminomycin |
Streptomyces sp. |
S-adenosyl-L-homocysteine + daunorubicinol |
- |
? |
2.1.1.292 | S-adenosyl-L-methionine + 13-dihydrocarminomycin |
- |
Streptomyces sp. |
S-adenosyl-L-homocysteine + 13-dihydrodaunomycin |
- |
? |