EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.260 | more |
Nep1 target site identification by mass spectrometry, isotope-labeling, and fluorescence anisotropy measurements |
Saccharomyces cerevisiae |
? |
- |
? |
2.1.1.260 | more |
RNA-binding specificity by in vitro using fluorescence quenching assays and yeast three-hybrid screening. Identification of the binding site for methylation target RNAs by NMR-spectroscopy, overview |
Homo sapiens |
? |
- |
? |
2.1.1.260 | more |
the Methanocaldococcus jannaschii Nep1 binds to the Saccharomyces cerevisiae Nep1 RNA consensus sequence 5'-UUCAAC-3' in the Methanocaldococcus jannaschii 16S rRNA. The region of Methanocaldococcus jannaschii 16S rRNA is equivalent to nt 1190-1195 in yeast 18S rRNA including the m1acp3-psi nucleotide at position 1191. And binding of MjNep1 to a 6mer RNA containing the consensus sequence 5'-UUCAAC-3'. Even a 5'-truncated RNA lacking the first uridine of the consensus sequence is bound with comparable affinity. RNA-binding specificity by in vitro using fluorescence quenching assays and yeast three-hybrid screening. Identification of the binding site for methylation target RNAs by NMR-spectroscopy, overview |
Methanocaldococcus jannaschii |
? |
- |
? |
2.1.1.260 | S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA |
- |
Homo sapiens |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA |
- |
? |
2.1.1.260 | S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA |
- |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA |
- |
? |
2.1.1.260 | S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA |
- |
Methanocaldococcus jannaschii |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA |
- |
? |
2.1.1.260 | S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA |
- |
Archaeoglobus fulgidus |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA |
- |
? |
2.1.1.260 | S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA |
- |
Methanocaldococcus jannaschii |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA |
product identification by 1H-NMR-spectroscopy |
? |
2.1.1.260 | S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA |
Nep1 catalyzes the psi1191 methylation in vivo. The in vivo target site for Nep1-catalyzed methylation is located within loop 35 of the 18S rRNA that contains the unique hypermodification of U1191 to 1-methyl-3-(3-amino-3-carboxypropyl)-pseudouridine |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA |
- |
? |
2.1.1.260 | S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA |
RNA-oligonucleotides corresponding to nucleotides 1245-1255 of human 18S rRNA and containing pseudouridine (5'-GACWCAACACG-3') at position 1248. This position corresponds to nucleotide 914 of Methanocaldococcus jannaschii 16S rRNA and to nt 1191 of yeast 18S rRNA |
Homo sapiens |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA |
product identification by MALDI-mass spectrometrical analysis |
? |