EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin |
a pH-dependent conformational change is required for methyl transfer in the forward and reverse directions, but this step is not rate-limiting |
Moorella thermoacetica |
a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin |
- |
r |
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin |
the reaction is performed by the intact gamma subunit and fragments of the delta subunit, components of the proteolyzed acetyl-CoA decarbonylase synthase, ACDS, complex, overview |
Methanosarcina barkeri |
a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin |
- |
r |
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin |
a pH-dependent conformational change is required for methyl transfer in the forward and reverse directions, but this step is not rate-limiting, the pH-dependent protein conformational change in the ternary complex is linked to deprotonation of the active complexes |
Moorella thermoacetica |
a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin |
- |
r |
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin |
structures of tetrahydrosarcinapterin and N5-methyl-tetrahydrosarcinapterin, overview. The reaction is performed by the intact gamma subunit and fragments of the delta subunit, components of the proteolyzed acetyl-CoA decarbonylase synthase, ACDS, complex, overview |
Methanosarcina barkeri |
a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin |
- |
r |
2.1.1.245 | CH3-H4SPt + cob(I)amide-protein + H+ |
- |
Methanosarcina barkeri |
H4SPt + CH3-cob(III)amide-protein |
- |
r |
2.1.1.245 | more |
reduction of the cobalt atom of the corrinoid to Co1+ by Ti(III)citrate, the gamma subunit CdhE contains the single 4Fe-4S center in the C/Fe-S enzyme |
Methanosarcina thermophila |
? |
- |
? |
2.1.1.245 | more |
the methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase catalyzes transfer of the N5-methyl group from (6S)-methyltetrahydrofolate, i.e. CH3-H4folate, to the cobalt center of a corrinoid/iron-sulfur protein, CFeSP, forming methylcob(III)-amide and H4folate |
Moorella thermoacetica |
? |
- |
? |