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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/1519 in 18S rRNA methylation of Escherichia coli 30S ribosomes. Under assay conditions the enzyme produces both N6-methyladenine and N6-dimethyladenine, with 0.8times as much N6-methyladenine as N6-dimethyladenine Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 18S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA - Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA 4 methyl groups are incorporated per 16S RNA molecule, both adenine residues in the 16S RNA sequence AACCUG are dimethylated Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA dimethylates the adenine residues in 16S rRNA-derived oligonucleotide with the specific sequence AACCUG Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA dimethylation of AI518 and A1519 in the hairpin loop of 16S rRNA. Site-specific mutagenesis of 16S rRNA of Escherichia coli ribosomes is used to make five mutations around the highly conserved UI512-GI523 base pair in the 3'-terminal hairpin. G1523 and C1524 in the stem are important determinants for the dimethylation of A1518 and AI519 in the loop Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA neither of the adenine residues is required for methylation of the other, ruling out any obligate order of methylation of A1518 and A1519. Mutation of either A1518 or A1519 to C, G or U has little effect on the ability of the mutant RNA to reconstitute a 30S ribosome containing a full complement of ribosomal proteins Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA recombinant KsgA is able to efficiently methylate 30S subunits isolated from strains of Escherichia coli resistant to kasugamycin, but not wild-type 30S subunits, indicating that the methylation function is specific for A1518 and A1519. KsgA is unable to utilize 30S subunits in the translationally active state as a substrate Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA binding of 30S subunit and S-adenosyl-L-methionine, structure, overview. After the S-adenosyl-L-methionine addition, KsgA dissociates rapidly from the subunits. The binding of KsgA to substrate is complex and requires regions of rRNA well beyond helix 45, including regions of the 790 loop Escherichia coli 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
Display the reaction diagram Show all sequences 2.1.1.1824 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA - ?
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