EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.175 | 2 S-adenosyl-L-methionine + tricetin |
stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). The enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin |
Hordeum vulgare |
2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin |
- |
? |
2.1.1.175 | 2 S-adenosyl-L-methionine + tricetin |
the enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin |
Zea mays |
2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin |
- |
? |
2.1.1.175 | 2 S-adenosyl-L-methionine + tricetin |
the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives. The enzyme also catalyzes the methylation of luteolin, myricetin (formation of the 3',5'-dimethyl ether of myricetin, EC 2.1.1.149) and caffeoyl-CoA. ROMT-15 exhibits similar Kcat/Km values for the four substrates. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity |
Oryza sativa |
2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin |
- |
? |
2.1.1.175 | 2 S-adenosyl-L-methionine + tricetin |
the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives. The enzyme also catalyzes the methylation of luteolin, myricetin (formation of the 3',5'-dimethyl ether of myricetin, EC 2.1.1.149) and caffeoyl-CoA. ROMT-17 prefers tricetin. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity |
Oryza sativa |
2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin |
- |
? |
2.1.1.175 | S-adenosyl-L-methionine + 3'-O-methyltricetin |
the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives |
Hordeum vulgare |
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin |
- |
? |
2.1.1.175 | S-adenosyl-L-methionine + 3'-O-methyltricetin |
the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives |
Zea mays |
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin |
- |
? |
2.1.1.175 | S-adenosyl-L-methionine + 3'-O-methyltricetin |
the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives |
Oryza sativa |
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin |
- |
? |
2.1.1.175 | S-adenosyl-L-methionine + selgin |
- |
Oryza sativa Japonica Group |
S-adenosyl-L-homocysteine + tricin |
- |
? |
2.1.1.175 | S-adenosyl-L-methionine + tricetin |
the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives |
Hordeum vulgare |
S-adenosyl-L-homocysteine + 3'-O-methyltricetin |
- |
? |
2.1.1.175 | S-adenosyl-L-methionine + tricetin |
the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives |
Zea mays |
S-adenosyl-L-homocysteine + 3'-O-methyltricetin |
- |
? |