EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.1.1.167 | more |
Spb1p is required for proper assembly of pre-ribosomal particles during the biogenesis of 60S ribosomal subunits |
Saccharomyces cerevisiae |
? |
- |
? |
| 2.1.1.167 | more |
a point mutation in the Ado-Met binding site of Spb1p affects cell growth but does not abolish methylation of U2918. When this mutation is combined with disruption of snR52 cell growth is severely impaired and U2918 is no longer methylated. In vitro, Spb1p is able to methylate U2918 on 60S subunits. For this methylation two mechanisms coexist: a site-specific methyltransferase (Spb1p) and a snoRNA-dependent mechanism |
Saccharomyces cerevisiae |
? |
- |
? |
| 2.1.1.167 | S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA |
Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA |
- |
? |
| 2.1.1.167 | S-adenosyl-L-methionine + uridine2918 in 25S rRNA |
the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA |
- |
? |
| 2.1.1.167 | S-adenosyl-L-methionine + uridine2918 in 25S rRNA |
no methylation of uridine2791 |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA |
- |
? |
