EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.5.99.B4 | more |
analysis of specificity of electron acceptors using 2,6-dichloroindophenol, 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+ |
Pseudomonas putida |
? |
- |
? |
1.5.99.B4 | more |
analysis of specificity of electron acceptors using 2,6-dichloroindophenol, 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+. Enzyme BjOdhAB2C and BjOdhAB1C both function as octopine-specific OpnDH, poor activity with nopaline |
Bradyrhizobium japonicum |
? |
- |
? |
1.5.99.B4 | more |
analysis of specificity of electron acceptors using 2,6-dichloroindophenol, 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+. Enzyme BjOdhAB2C and BjOdhAB1C both function as octopine-specific OpnDH, poor activity with nopaline |
Bradyrhizobium japonicum USDA110 |
? |
- |
? |
1.5.99.B4 | more |
analysis of specificity of electron acceptors using 2,6-dichloroindophenol, 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+ |
Pseudomonas putida KT 2240 |
? |
- |
? |
1.5.99.B4 | N2-(D-1,3-dicarboxypropyl)-L-arginine + FAD + H2O |
i.e. nopaline |
Pseudomonas putida |
L-arginine + 2-oxoglutarate + FADH2 |
- |
? |
1.5.99.B4 | N2-(D-1,3-dicarboxypropyl)-L-arginine + FAD + H2O |
i.e. nopaline |
Pseudomonas putida KT 2240 |
L-arginine + 2-oxoglutarate + FADH2 |
- |
? |
1.5.99.B4 | N2-(D-1-carboxyethyl)-L-arginine + FAD + H2O |
i.e. D-octopine |
Pseudomonas putida |
L-arginine + pyruvate + FADH2 |
- |
? |
1.5.99.B4 | N2-(D-1-carboxyethyl)-L-arginine + FAD + H2O |
i.e. D-octopine |
Bradyrhizobium japonicum |
L-arginine + pyruvate + FADH2 |
- |
? |
1.5.99.B4 | N2-(D-1-carboxyethyl)-L-arginine + FAD + H2O |
i.e. D-octopine, the electrons obtained through the oxidation of octopine are received by FMN via FAD in the beta-subunit, and finally transferred to an electron acceptor via the [2Fe-2S] cluster bound to [Fe-S]site 2, FAD in the alpha-subunit, and/or [4Fe-4S] cluster bound to [Fe-S]site 1. 2,6-Dichloroindophenol is used as final electron acceptor |
Bradyrhizobium japonicum |
L-arginine + pyruvate + FADH2 |
- |
? |
1.5.99.B4 | N2-(D-1-carboxyethyl)-L-arginine + FAD + H2O |
i.e. D-octopine |
Bradyrhizobium japonicum USDA110 |
L-arginine + pyruvate + FADH2 |
- |
? |