EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.5.1.49 | DELTA1-pyrroline-2-carboxylate + NADH + H+ |
the enzyme is part of the trans-3-hydroxy-L-proline pathway |
Colwellia psychrerythraea |
L-proline + NAD+ |
- |
? |
1.5.1.49 | DELTA1-pyrroline-2-carboxylate + NADH + H+ |
preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value |
Colwellia psychrerythraea |
L-proline + NAD+ |
- |
? |
1.5.1.49 | DELTA1-pyrroline-2-carboxylate + NADPH + H+ |
the enzyme is part of the trans-3-hydroxy-L-proline pathway |
Colwellia psychrerythraea |
L-proline + NADP+ |
- |
? |
1.5.1.49 | DELTA1-pyrroline-2-carboxylate + NADPH + H+ |
preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value |
Colwellia psychrerythraea |
L-proline + NADP+ |
- |
? |
1.5.1.49 | L-proline + NAD+ |
preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 |
Colwellia psychrerythraea |
DELTA1-pyrroline-2-carboxylate + NADH + H+ |
- |
r |
1.5.1.49 | L-proline + NADP+ |
preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 |
Colwellia psychrerythraea |
DELTA1-pyrroline-2-carboxylate + NADPH + H+ |
- |
r |
1.5.1.49 | more |
no significant activity with DELTA1-piperideine-2-carboxylate or L-pipecolate. The kcat/Km values for DELTA1-piperideine-2-carboxylate and L-pipecolate in the presence of NADP+(H) are 111fold and 223fold lower than those for DELTA1-pyrroline-2-carboxylate and L-proline, mainly caused by 90fold and 51fold lower kcat values, respectively |
Colwellia psychrerythraea |
? |
- |
? |