EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.3.99.31 | 15-cis-phytoene + 3 acceptor |
- |
Rubrivivax gelatinosus |
all-trans-neurosporene + 3 reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + 3 acceptor |
the enzyme simultaneously catalyzes a three- and four-step desaturation of phytoene producing both neurosporene and lycopene. These carotenes are intermediates for the synthesis of spheroidene and spirilloxanthin, respectively |
Rubrivivax gelatinosus |
all-trans-neurosporene + 3 reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + 3 acceptor |
the enzyme simultaneously catalyzes a three- and four-step desaturation of phytoene producing both neurosporene and lycopene. The ratio of lycopene to neurosporene of the wild-type enzyme under assay conditions is 88:12 after complementation of CrtI in pUC8(DH5a/pACCrtEBEU/pUCCrtIRg) and 26:74 after complementation of CrtI in pPEU(DH5a/pACCrtEBEU/pPEUCrtIRg). The affinity for neurosporene conversion is poorer than for phytoene conversion. This explains the formation of two desaturation products |
Rubrivivax gelatinosus |
all-trans-neurosporene + 3 reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + 4 acceptor |
- |
Rubrivivax gelatinosus |
all-trans-lycopene + 4 reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + 4 acceptor |
overall reaction. all-trans-Lycopene is the principal desaturase product constituting 60% of the total reaction products. The desaturase intermediates all-trans-phytofluene and all-trans-zeta-carotene are also detected and constitute 27 and 12% of the total desaturase products formed |
Pantoea ananatis |
all-trans-lycopene + 4 reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + 4 acceptor |
the enzyme simultaneously catalyzes a three- and four-step desaturation of phytoene producing both neurosporene and lycopene. These carotenes are intermediates for the synthesis of spheroidene and spirilloxanthin, respectively |
Rubrivivax gelatinosus |
all-trans-lycopene + 4 reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + 4 acceptor |
the enzyme simultaneously catalyzes a three- and four-step desaturation of phytoene producing both neurosporene and lycopene. The ratio of lycopene to neurosporene of the wild-type enzyme under assay conditions is 88:12 after complementation of CrtI in pUC8(DH5a/pACCrtEBEU/pUCCrtIRg) and 26:74 after complementation of CrtI in pPEU(DH5a/pACCrtEBEU/pPEUCrtIRg). The affinity for neurosporene conversion is poorer than for phytoene conversion. This explains the formation of two desaturation products |
Rubrivivax gelatinosus |
all-trans-lycopene + 4 reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + acceptor |
- |
Rhodospirillum rubrum |
all-trans-phytofluene + reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + acceptor |
- |
Pantoea ananatis |
all-trans-phytofluene + reduced acceptor |
- |
? |
1.3.99.31 | 15-cis-phytoene + acceptor |
- |
Cereibacter azotoformans |
all-trans-phytofluene + reduced acceptor |
- |
? |